| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.220844 |
| Chromosome: | chromosome 10 |
| Location: | 606720 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g421900 | SRR27 | Scavenger receptor cysteine rich (SRCR) protein; (1 of 5) 1.4.3.13 - Protein-lysine 6-oxidase / Lysyl oxidase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGTACCGCAGCTGGACCTGCGGCTCCGTC |
| Internal bar code: | CAGCCTGGGAGTGATAGTGTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 688 |
| LEAP-Seq percent confirming: | 99.7388 |
| LEAP-Seq n confirming: | 7636 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCTCCGTGACAAAGAAGAG |
| Suggested primer 2: | ATTGGCGTGTTCATCGTGTA |