Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.220862 |
Chromosome: | chromosome_9 |
Location: | 7032380 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre09.g411200 | TEF5 | Rieske [2Fe-2S] domain containing protein | antisense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TCTGCTAGCGGACGTCTGCGCCAAGTCCAA |
Internal bar code: | TGTTGCTGCCAGGATGCCGTA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 892 |
LEAP-Seq percent confirming: | 99.7024 |
LEAP-Seq n confirming: | 5026 |
LEAP-Seq n nonconfirming: | 15 |
LEAP-Seq n unique pos: | 37 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TATGATAAGCGTGCGACTGC |
Suggested primer 2: | CCTTCGTGGAGTTGGTTGTT |