Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.220867 |
Chromosome: | chromosome_2 |
Location: | 2418042 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre02.g091500 | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TTCGATGCAGTGGTGCCTGTAGGTGCTCGG |
Internal bar code: | CGGTTGCGCGATATTGAAGAAG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 833 |
LEAP-Seq percent confirming: | 96.2327 |
LEAP-Seq n confirming: | 3474 |
LEAP-Seq n nonconfirming: | 136 |
LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TCACCGCTCACTTGTTTGTC |
Suggested primer 2: | GGAGCGAGGCTCAATGTAAG |