| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.220883 |
| Chromosome: | chromosome 3 |
| Location: | 1958182 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g155700 | (1 of 2) PTHR22840//PTHR22840:SF12 - FAMILY NOT NAMED // WD REPEAT-CONTAINING PROTEIN 36 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGAGCGGCGCCAGCACGGCCAGGATGGTGC |
| Internal bar code: | CGTTTCGAAAGGTCCGGTTGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 332 |
| LEAP-Seq percent confirming: | 99.7231 |
| LEAP-Seq n confirming: | 13324 |
| LEAP-Seq n nonconfirming: | 37 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCGATGCTTTGGATTTAGG |
| Suggested primer 2: | AGTCTTGGGACCACACCTTG |