| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.220896 |
| Chromosome: | chromosome 16 |
| Location: | 2695515 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g662350 | TSPS1,TPS2 | (1 of 3) 2.4.1.15 - Alpha,alpha-trehalose-phosphate synthase (UDP-forming) / UDP-glucose--glucose-phosphate glucosyltransferase; Trehalose-6-phosphate synthase, class I | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTTTGCAGTTAAGGCCGAGGCCGAATCCC |
| Internal bar code: | TCGCGCCCTAGGAAAAGGGTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 480 |
| LEAP-Seq percent confirming: | 94.3414 |
| LEAP-Seq n confirming: | 3918 |
| LEAP-Seq n nonconfirming: | 235 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACGAAGCGTTTGTGTTTGC |
| Suggested primer 2: | GCCGTAGCTCTCATAGTGGC |