| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.220898 |
| Chromosome: | chromosome 12 |
| Location: | 6528268 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g538850 | (1 of 1) PTHR13720//PTHR13720:SF9 - WD-40 REPEAT PROTEIN // ECHINODERM MICROTUBULE-ASSOCIATED PROTEIN-LIKE ELP-1; Similar to Echinoderm Microtubule Associated Protein Like 5 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATCCGCTGTGCCGCCTCCTCGGCCGCGAC |
| Internal bar code: | GTGAATTCCGGCCCTGGCGCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 130 |
| LEAP-Seq percent confirming: | 99.5215 |
| LEAP-Seq n confirming: | 1248 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGAACTCCTCCCAGCTCAC |
| Suggested primer 2: | CGTTATCGTCACCACGTGTC |