| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.220912 |
| Chromosome: | chromosome 12 |
| Location: | 2513688 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g506250 | HTA18 | Histone H2A; (1 of 30) K11251 - histone H2A (H2A) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCTGAGGTGCTGGAGCTGGCGGGCAACGC |
| Internal bar code: | TCCCTCTTTAATTGGTACGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1101 |
| LEAP-Seq percent confirming: | 95.8333 |
| LEAP-Seq n confirming: | 3542 |
| LEAP-Seq n nonconfirming: | 154 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGCAAAAAGCTGGTCTTG |
| Suggested primer 2: | CTTCTTGGAGGGCTCCTTCT |