| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.220914 |
| Chromosome: | chromosome 17 |
| Location: | 2249585 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g713600 | SUB13 | (1 of 14) 3.4.21.62 - Subtilisin; Subtilisin-like protease | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGGCCTGCGCCGCGACAGGGGGCAGGCAG |
| Internal bar code: | TGCGGGCGGGCCCTCGGCGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 312 |
| LEAP-Seq percent confirming: | 98.213 |
| LEAP-Seq n confirming: | 1319 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATTGAAGAGCTACCGCCTG |
| Suggested primer 2: | GGCTAGGAAGACAGTGGCTG |