| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.220920 |
| Chromosome: | chromosome 10 |
| Location: | 58081 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g417850 | (1 of 1) IPR003613//IPR011047 - U box domain // Quinonprotein alcohol dehydrogenase-like superfamily | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTGTGACGCATTGGGCGTACGGGCTTGC |
| Internal bar code: | TAGTCCCCCGCTGAGTCTCAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 530 |
| LEAP-Seq percent confirming: | 99.804 |
| LEAP-Seq n confirming: | 11203 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGACTGCTAGATAGGCACC |
| Suggested primer 2: | CGGTGGCCAGTAGTAGGTGT |