| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.220921 |
| Chromosome: | chromosome_11 |
| Location: | 2537718 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre11.g476050 | ODA2,DHC15,DYHG | Flagellar outer arm dynein heavy chain gamma | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AAGATGGTGTCGCTGTCCTCCTCCTTGGGC |
| Internal bar code: | CGCACGGTTCGACTCAAGGCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 692 |
| LEAP-Seq percent confirming: | 98.9048 |
| LEAP-Seq n confirming: | 5057 |
| LEAP-Seq n nonconfirming: | 56 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATCTACGTGTCGGATGTGG |
| Suggested primer 2: | GGGGTGCAAGTGGAAAAGTA |