Insertion junction: LMJ.RY0402.220924_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.220924
Chromosome:chromosome 3
Location:5366853
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre03.g184400 (1 of 3) 3.6.1.22//3.6.1.55 - NAD(+) diphosphatase / NADP pyrophosphatase // 8-oxo-dGTP diphosphatase / 8-oxo-dGTPase; Putative NAD+ diphosphatase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATACTCAATCGCAGCTCACAGCAGCTGCTG
Internal bar code:GAAACGGTCAGACGCCCACGAT

Confirmation - LEAP-Seq

LEAP-Seq distance:948
LEAP-Seq percent confirming:99.1479
LEAP-Seq n confirming:5934
LEAP-Seq n nonconfirming:51
LEAP-Seq n unique pos:42

Suggested primers for confirmation by PCR

Suggested primer 1:AACACGCACGTTGACTTCAG
Suggested primer 2:GTGAGTAGAAAAGCTGGGCG