| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.220927 |
| Chromosome: | chromosome 10 |
| Location: | 372288 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g420500 | (1 of 6) PTHR10117 - TRANSIENT RECEPTOR POTENTIAL CHANNEL | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCCGAGTCCAAGTTCTCGCTGGCGGTCA |
| Internal bar code: | GTTCCTTTGCAAGGCGTATTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 823 |
| LEAP-Seq percent confirming: | 99.4121 |
| LEAP-Seq n confirming: | 3382 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCACCCTGGACAAACAGT |
| Suggested primer 2: | GATCATGGGTTGGGTTTTTG |