| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.220956 |
| Chromosome: | chromosome 6 |
| Location: | 1452846 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g259401 | SMM16 | S-adenosyl-L-methionine-dependent methyltransferase; (1 of 2) PTHR10108//PTHR10108:SF813 - METHYLTRANSFERASE // SUBFAMILY NOT NAMED | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGAAAGATTGGAGGGAACAGAGTGTACCG |
| Internal bar code: | GACCGCAGCCTAATGGCGCGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 969 |
| LEAP-Seq percent confirming: | 99.0837 |
| LEAP-Seq n confirming: | 26493 |
| LEAP-Seq n nonconfirming: | 245 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATGGTGGTGTTGAGGAGTG |
| Suggested primer 2: | ATCACTCGCAACTAACGCCT |