| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.221004 |
| Chromosome: | chromosome 16 |
| Location: | 1091839 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g649700 | (1 of 4) 2.1.1.127 - [Ribulose-bisphosphate carboxylase]-lysine N-methyltransferase / RuBisCO methyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGCTTGAAAGTGTGGGGCGGACAGTTTGA |
| Internal bar code: | GGGTCGTACTCGCTTAGTATGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 567 |
| LEAP-Seq percent confirming: | 99.3974 |
| LEAP-Seq n confirming: | 10226 |
| LEAP-Seq n nonconfirming: | 62 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTCGGCCAACGACTAGAAG |
| Suggested primer 2: | ATGATGTCTTCTTGGGTCGG |