| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.221012 |
| Chromosome: | chromosome 9 |
| Location: | 4663143 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g396735 | (1 of 1) PTHR18895:SF3 - METHYLTRANSFERASE-LIKE PROTEIN 20 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCTCACCACGCTGGCCGCGCCGTACTTGA |
| Internal bar code: | CTTATTGCTCTAACATCGGTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 435 |
| LEAP-Seq percent confirming: | 99.9469 |
| LEAP-Seq n confirming: | 1882 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTGAAATGGCGAGTGTATG |
| Suggested primer 2: | CGTCTTGACCACGAAGTCCT |