| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.221036 |
| Chromosome: | chromosome 12 |
| Location: | 5950080 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g534500 | (1 of 1) PTHR20955//PTHR20955:SF1 - UNCHARACTERIZED // PROTEIN JAGUNAL HOMOLOG 1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGTTGCAGGTCCCGGCCATCACCAAGGG |
| Internal bar code: | TAAGAAAGTCTCAGGTATCCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 826 |
| LEAP-Seq percent confirming: | 99.7797 |
| LEAP-Seq n confirming: | 1359 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGTAGGCCCGTAACGAAAC |
| Suggested primer 2: | CACTCTGAGGTTGGTTGGGT |