| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.221038 |
| Chromosome: | chromosome 16 |
| Location: | 6817387 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g674964 | (1 of 1) K06911 - uncharacterized protein (K06911) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCAACCCGCAACCGCAACATGCTCACCGC |
| Internal bar code: | TCGAGCTCGCGAACGATGGGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 484 |
| LEAP-Seq percent confirming: | 98.9151 |
| LEAP-Seq n confirming: | 2553 |
| LEAP-Seq n nonconfirming: | 28 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCATATGTGAATGGCTGTGC |
| Suggested primer 2: | TCATGTCGCGTAGCAAAGTC |