| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.221062 |
| Chromosome: | chromosome 7 |
| Location: | 535577 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g316200 | (1 of 726) IPR011009 - Protein kinase-like domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCGGTGTCGTCCTCGTGTCGTCCTCACAC |
| Internal bar code: | TTCACTGGTTAGGCCGTAATTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 514 |
| LEAP-Seq percent confirming: | 96.5355 |
| LEAP-Seq n confirming: | 4514 |
| LEAP-Seq n nonconfirming: | 162 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTATTAGAGCGTCGGGGGT |
| Suggested primer 2: | TGGCGAGCTGCCTTAGTTAT |