Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.221089 |
Chromosome: | chromosome_6 |
Location: | 4067962 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre06.g278212 | CGL46 | Predicted protein | antisense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TCCAGGTCGCGCTCGCCGATGCAGTGCTCC |
Internal bar code: | CCGGGGCCTCGCTACAGAGAAG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 642 |
LEAP-Seq percent confirming: | 85.4528 |
LEAP-Seq n confirming: | 2661 |
LEAP-Seq n nonconfirming: | 453 |
LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TCGGCTAAGCTTTCAATCGT |
Suggested primer 2: | TCTTGGCTGACTCATTGCTG |