| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.221095 |
| Chromosome: | chromosome 3 |
| Location: | 5259618 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g183250 | (1 of 1) IPR027437 - 30s ribosomal protein S13, C-terminal | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCGCTGTTGCCCGGACCATATTTGGGACC |
| Internal bar code: | AACATAGGCCTGTCGGAATTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 705 |
| LEAP-Seq percent confirming: | 99.6772 |
| LEAP-Seq n confirming: | 10500 |
| LEAP-Seq n nonconfirming: | 34 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATATGGTTGTTTGGCAACG |
| Suggested primer 2: | AAAGCAAAACTTGCGGAGAA |