| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.221112 |
| Chromosome: | chromosome_12 |
| Location: | 8412232 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre12.g548650 | BBS4 | Bardet Biedl syndrome-4 associated protein | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ATTTTGGCTTCCAATTTGGGTTTGGTTCTT |
| Internal bar code: | GTGTTCGGGGTTCACCGGGTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 249 |
| LEAP-Seq percent confirming: | 91.5638 |
| LEAP-Seq n confirming: | 15727 |
| LEAP-Seq n nonconfirming: | 1449 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAGTGTGGTGAGCTTTTGC |
| Suggested primer 2: | TCAAGCAGTACGACAGGTGC |