Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.221112 |
Chromosome: | chromosome_12 |
Location: | 8412246 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre12.g548650 | BBS4 | Bardet Biedl syndrome-4 associated protein | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TCGCATCCAACCCCTTCTCGCCCCTCTGCA |
Internal bar code: | GACCGTCACACCGCACTCCGAG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 625 |
LEAP-Seq percent confirming: | 5.28036 |
LEAP-Seq n confirming: | 937 |
LEAP-Seq n nonconfirming: | 16808 |
LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TGAGTGTGGTGAGCTTTTGC |
Suggested primer 2: | TCAAGCAGTACGACAGGTGC |