| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.221142 |
| Chromosome: | chromosome 9 |
| Location: | 2109143 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g393700 | MMP3 | Matrix metalloproteinase; (1 of 2) IPR016517 - Peptidase M11, autolysin | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTTGAAGCTACTGGTGATGCCGGAGTCGT |
| Internal bar code: | ATGCGTTTTACACGAAGGACTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 753 |
| LEAP-Seq percent confirming: | 97.8261 |
| LEAP-Seq n confirming: | 1980 |
| LEAP-Seq n nonconfirming: | 44 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACAACTACGGACTGGAGCA |
| Suggested primer 2: | CACCGACTTCGTTAAACGGT |