| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.221150 |
| Chromosome: | chromosome_17 |
| Location: | 6007376 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre17.g740700 | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTGCGTGCTAGCCATGCTCGCCAGCCTCAT |
| Internal bar code: | GACTTGGCGTATATACGATGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 673 |
| LEAP-Seq percent confirming: | 81.0196 |
| LEAP-Seq n confirming: | 13890 |
| LEAP-Seq n nonconfirming: | 3254 |
| LEAP-Seq n unique pos: | 54 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATCACTCAGGAGGATCGGG |
| Suggested primer 2: | TGCTGGTGTCTCAAAAGTGC |