Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.221150 |
Chromosome: | chromosome_17 |
Location: | 6007381 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre17.g740700 | antisense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CAGATGCCGTGGGCGGTAGGCGCACAGGCT |
Internal bar code: | TGGCATACTACGAGACATGAAA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 960 |
LEAP-Seq percent confirming: | 99.7632 |
LEAP-Seq n confirming: | 3371 |
LEAP-Seq n nonconfirming: | 8 |
LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TGCTGGTGTCTCAAAAGTGC |
Suggested primer 2: | TATCACTCAGGAGGATCGGG |