Insertion junction: LMJ.RY0402.221151_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.221151
Chromosome:chromosome 17
Location:5746277
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre17.g739150 CTPA5,TSP5 (1 of 8) 3.4.21.102 - C-terminal processing peptidase / Tsp protease; putative C-terminal peptidase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCCAATCCCAAAACAAACCATTGTCAACC
Internal bar code:ACCACCTTTTGTATCTTACATC

Confirmation - LEAP-Seq

LEAP-Seq distance:482
LEAP-Seq percent confirming:97.6654
LEAP-Seq n confirming:251
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GGTCCATTAAATCCTCCCGT
Suggested primer 2:CGTCTACCCTACACCTCCCA