| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.221159 |
| Chromosome: | chromosome 17 |
| Location: | 6233714 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g742200 | (1 of 1) PTHR22870:SF220 - RCC1 AND BTB DOMAIN-CONTAINING PROTEIN 2 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCAAACTCACGATGGCATTGTGCGGCAC |
| Internal bar code: | GAGTGTTCAACAGAGATTTTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1161 |
| LEAP-Seq percent confirming: | 99.3251 |
| LEAP-Seq n confirming: | 26490 |
| LEAP-Seq n nonconfirming: | 180 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCGTACTGGGACAGATGTT |
| Suggested primer 2: | CGAGTAACAAGAGGGCAAGC |