| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.221178 |
| Chromosome: | chromosome 9 |
| Location: | 1726523 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g396700 | ACK1 | (1 of 2) 2.7.2.1 - Acetate kinase / AK; Acetate kinase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGCGAGGATGTCGCTGGCAAGATCGAGAC |
| Internal bar code: | CGTAGCGCCGGGTGAGGTTGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1 |
| LEAP-Seq percent confirming: | 98.2885 |
| LEAP-Seq n confirming: | 804 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGCTGGGTGTTGGTTTAT |
| Suggested primer 2: | GGGAGAATTCCATTGCTGAA |