| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.221179 |
| Chromosome: | chromosome 4 |
| Location: | 477114 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g217929 | (1 of 1) 1.8.2.1//1.8.3.1 - Sulfite dehydrogenase // Sulfite oxidase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTTCAATGAGTTGCGAAGTGCTTAAACGT |
| Internal bar code: | ATTGGTTTCGGTCATAACAGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 536 |
| LEAP-Seq percent confirming: | 96.9427 |
| LEAP-Seq n confirming: | 761 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTATATGCCCGAGCCCAGTA |
| Suggested primer 2: | GCGTTTCTTTCGTAGGCTTG |