| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.221216 |
| Chromosome: | chromosome 6 |
| Location: | 3588905 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278121 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGGTCAATCTGCTGATGCATACTGTCCA |
| Internal bar code: | ACAGTCCGAATGTGGTGAAGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 989 |
| LEAP-Seq percent confirming: | 98.6691 |
| LEAP-Seq n confirming: | 69316 |
| LEAP-Seq n nonconfirming: | 935 |
| LEAP-Seq n unique pos: | 197 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAAAATACCGTACAGCCGCC |
| Suggested primer 2: | TTCAGTCATCACAACGGGAA |