| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.221231 |
| Chromosome: | chromosome 16 |
| Location: | 4623415 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g687600 | (1 of 7) IPR000104//IPR020683 - Antifreeze protein, type I // Ankyrin repeat-containing domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTCTTCCAACACCCGCCCAGCCGCCGCCC |
| Internal bar code: | AACTTGGCTCTGGGTAGACCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 863 |
| LEAP-Seq percent confirming: | 98.7199 |
| LEAP-Seq n confirming: | 32005 |
| LEAP-Seq n nonconfirming: | 415 |
| LEAP-Seq n unique pos: | 132 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTACGCCTCCCGTTTTGTA |
| Suggested primer 2: | TGCGAGATCGAGGTCAATAA |