| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.221238 |
| Chromosome: | chromosome 6 |
| Location: | 7746037 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g302100 | (1 of 1) PTHR12907//PTHR12907:SF10 - EGL NINE HOMOLOG-RELATED // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAGATGGGAGGCCAGGTGGCAGGGAGTAG |
| Internal bar code: | CCCTGACACCGTGTCCATGCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 369 |
| LEAP-Seq percent confirming: | 97.7664 |
| LEAP-Seq n confirming: | 3239 |
| LEAP-Seq n nonconfirming: | 74 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTACTTGGGAGCGAGTGAGG |
| Suggested primer 2: | TGCAGTTACGAGAATGTCGG |