| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.221247 |
| Chromosome: | chromosome 12 |
| Location: | 3147336 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g499500 | SAC3,SNRK2B | snRK1 family in Chlamydomonas, subgroup 2; (1 of 1) PTHR24343:SF207 - SERINE/THREONINE-PROTEIN KINASE SRK2C | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCTTGTATACTGCCTGCGGGAGCGCTTCA |
| Internal bar code: | AGAGTGCATTCCGTCAAGGTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 809 |
| LEAP-Seq percent confirming: | 99.1731 |
| LEAP-Seq n confirming: | 1919 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAAGGCCACTCCAGCCATA |
| Suggested primer 2: | AAGTGGTACCTCAAGGGGCT |