| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.221250 |
| Chromosome: | chromosome 17 |
| Location: | 1956694 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g710800 | NFU3,NIFU3 | (1 of 3) PF01106 - NifU-like domain (NifU); iron-sulfur cluster assembly protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCGGCTGTAGCCCACGTACGCCGCCGCC |
| Internal bar code: | ATGTTACTCATAACGGCAATGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1089 |
| LEAP-Seq percent confirming: | 95.9603 |
| LEAP-Seq n confirming: | 11901 |
| LEAP-Seq n nonconfirming: | 501 |
| LEAP-Seq n unique pos: | 48 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAAAAGAAGGGCAGTACGA |
| Suggested primer 2: | CACGAGCAGGATGCTTACAA |