| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.221255 |
| Chromosome: | chromosome 12 |
| Location: | 4729579 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g523700 | (1 of 10) IPR000104//IPR001810 - Antifreeze protein, type I // F-box domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCCCTGACCTAACAAATCCGTTACGTTAC |
| Internal bar code: | ACGCTGACTCAGTAACTGGTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 518 |
| LEAP-Seq percent confirming: | 78.9308 |
| LEAP-Seq n confirming: | 251 |
| LEAP-Seq n nonconfirming: | 67 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCTGTCGCTCGTCTTCTAC |
| Suggested primer 2: | CTGGGTCGGTCGTCAACTAT |