Insertion junction: LMJ.RY0402.221265_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.221265
Chromosome:chromosome 3
Location:2528333
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre03.g160000 HDA3 (1 of 3) PTHR10625//PTHR10625:SF102 - HISTONE DEACETYLASE // HISTONE DEACETYLASE 10; RPD3/HDA1 type histone deacetylase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCATACCGCACCCTTGCGCGCTCTGCGTC
Internal bar code:CTCTCGCGAGAGAGGACCTATA

Confirmation - LEAP-Seq

LEAP-Seq distance:655
LEAP-Seq percent confirming:99.6365
LEAP-Seq n confirming:6031
LEAP-Seq n nonconfirming:22
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:GACTCTTACGGGGATGACGA
Suggested primer 2:GAGACACGTGCTGACGGTAA