| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.221266 |
| Chromosome: | chromosome_1 |
| Location: | 1773832 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g009550 | NAT6 | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTCAAGGTGCGGGCTGGGGGCTGTGGGTTA |
| Internal bar code: | GGGGGAGCCTGCGCCCCAGAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 643 |
| LEAP-Seq percent confirming: | 98.7677 |
| LEAP-Seq n confirming: | 2164 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGATGGACGAGTGTGACGC |
| Suggested primer 2: | CTCGTCGTGAATCCCTGAAT |