| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.221277 |
| Chromosome: | chromosome 16 |
| Location: | 5901609 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g677150 | (1 of 2) 2.4.1.122 - Glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGCGGGGAGTGCGGTTTTGGGGAAGTTCG |
| Internal bar code: | ATGTACAGTAATGTGGGTAGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 874 |
| LEAP-Seq percent confirming: | 99.4258 |
| LEAP-Seq n confirming: | 5541 |
| LEAP-Seq n nonconfirming: | 32 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTGAGTTGTCATGCTTGCG |
| Suggested primer 2: | GGCCCCACCTTGATTTATTT |