| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.221281 |
| Chromosome: | chromosome 1 |
| Location: | 5622171 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g039950 | MFT32 | Major facilitator superfamily transporter; (1 of 1) PF06813 - Nodulin-like (Nodulin-like) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGAGAGTGAGAGAATGGGAGTGAGGCATGG |
| Internal bar code: | GTGACGTTCCGATGCCGCGATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 738 |
| LEAP-Seq percent confirming: | 97.2266 |
| LEAP-Seq n confirming: | 1858 |
| LEAP-Seq n nonconfirming: | 53 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCACTTTGGCAGCAACTACA |
| Suggested primer 2: | TTTTGCAGAGTTATGCGTCG |