| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.221301 |
| Chromosome: | chromosome 9 |
| Location: | 170851 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g386737 | (1 of 8) PF01697 - Glycosyltransferase family 92 (Glyco_transf_92) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGAACGGTGTATTGGGGCTCGCGCTGAGA |
| Internal bar code: | AGAACAAACTACGTATCGAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 309 |
| LEAP-Seq percent confirming: | 62.1053 |
| LEAP-Seq n confirming: | 177 |
| LEAP-Seq n nonconfirming: | 108 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTGTAACCCACAGCCAGTT |
| Suggested primer 2: | AGTTCGTGTACATAGGCGGC |