| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.221309 |
| Chromosome: | chromosome 9 |
| Location: | 3472035 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g389467 | OPR8 | (1 of 7) IPR014721 - Ribosomal protein S5 domain 2-type fold, subgroup; OctotricoPeptide Repeat protein 8 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCACAATCAGGCAGGCCGGTTTTATTCTTT |
| Internal bar code: | GACGACGGCTTCAGGTCGTTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 395 |
| LEAP-Seq percent confirming: | 89.1954 |
| LEAP-Seq n confirming: | 4656 |
| LEAP-Seq n nonconfirming: | 564 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGTGCAGACGTGTGTCAGG |
| Suggested primer 2: | TGACGAGCACTATCACTCCG |