| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.221345 |
| Chromosome: | chromosome 3 |
| Location: | 5240661 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g183000 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCAGGAGTGTCAACACCAGAGTTCGGTCT |
| Internal bar code: | TACGTGCGCCAGATTCACATGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 888 |
| LEAP-Seq percent confirming: | 99.6627 |
| LEAP-Seq n confirming: | 4137 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACATGGCTACGCCAGGTAAC |
| Suggested primer 2: | GTGGTCCTTCATCACGTCCT |