| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.221355 |
| Chromosome: | chromosome 9 |
| Location: | 4483294 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g395769 | FAP177 | Flagellar Associated Protein 177 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGAAGCGCCAGCGCAGCGCAAGCGTAACC |
| Internal bar code: | TTTTCCCTTCCTTACTGCCCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 237 |
| LEAP-Seq percent confirming: | 95.7251 |
| LEAP-Seq n confirming: | 1142 |
| LEAP-Seq n nonconfirming: | 51 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGTCTTGCTCGCATTATCG |
| Suggested primer 2: | AAATCGAGTAACCGGCACAC |