| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.221356 |
| Chromosome: | chromosome 3 |
| Location: | 1261056 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g150050 | (1 of 6) 3.1.3.4 - Phosphatidate phosphatase / Phosphatidic acid phospphatase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACACCCGCACCAGCGTCACCGCCGCCATG |
| Internal bar code: | CGCGTGCCTGTAGGTCGGGACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 66 |
| LEAP-Seq percent confirming: | 97.2222 |
| LEAP-Seq n confirming: | 140 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACCTACGTAGACCTGGCG |
| Suggested primer 2: | GCTATTGCCTTGCAGCCTAC |