Insertion junction: LMJ.RY0402.221370_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.221370
Chromosome:chromosome 2
Location:2719658
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g093600 TEH3 (1 of 6) PF03061 - Thioesterase superfamily (4HBT); Thioesterase-like protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTAGATAGGTTACATGGTACACGGTACAA
Internal bar code:CGGATGGTTCCCACGTCCGTTA

Confirmation - LEAP-Seq

LEAP-Seq distance:614
LEAP-Seq percent confirming:99.2141
LEAP-Seq n confirming:6312
LEAP-Seq n nonconfirming:50
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:AAGCATTCTTGCGCTCTGAT
Suggested primer 2:CAGTGAGTCCTAGCCAAGCC