| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.221402 |
| Chromosome: | chromosome 9 |
| Location: | 4517553 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g395917 | (1 of 1) 2.3.1.32 - Lysine N-acetyltransferase / Lysine N(6)-acetyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGCGGTAGATAGATACAGATGTGGGGCAA |
| Internal bar code: | CTGACTATCATCAGAACTTAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 354 |
| LEAP-Seq percent confirming: | 99.4709 |
| LEAP-Seq n confirming: | 3760 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCACAGAAGGCAGGTAGGCT |
| Suggested primer 2: | CACGCACAATACTGAATGGG |