| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.221405 |
| Chromosome: | chromosome 11 |
| Location: | 697402 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467624 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGTGTTGCGGCTGCTGCAGGGGGTAACC |
| Internal bar code: | CTACTGCTGGATAGGCTTCAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 278 |
| LEAP-Seq percent confirming: | 57.3679 |
| LEAP-Seq n confirming: | 619 |
| LEAP-Seq n nonconfirming: | 460 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAACAAGCACATCAACACC |
| Suggested primer 2: | GTGGTACTGGTGAGGCAGGT |