| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.221431 |
| Chromosome: | chromosome 13 |
| Location: | 805768 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g567100 | GLDH1,GLDH,ALO1 | (1 of 1) 1.3.2.3 - L-galactonolactone dehydrogenase / L-galactono-gamma-lactone dehydrogenase; L-galactono-1%252C4-lactone dehydrogenase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACTATTCGCTCCCCACCCTCCTCGCTCCA |
| Internal bar code: | GGTTCACCTTACGCAGTTTACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 117 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 167 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATGTAGCCACTCCGCTCTC |
| Suggested primer 2: | AGCGACAGGTGAGCTAGAGG |