Insertion junction: LMJ.RY0402.221443_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.221443
Chromosome:chromosome 2
Location:1372946
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre02.g083180 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGTGCCAGCTTACCCGACCGTGCCCCGGC
Internal bar code:GTAAGACCAACTCCGGGGGGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:713
LEAP-Seq percent confirming:50.068
LEAP-Seq n confirming:368
LEAP-Seq n nonconfirming:367
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:GTCATCAACGCCTGACACAG
Suggested primer 2:GTGTTGGGAAAGGTGGTGAT