| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.221448 |
| Chromosome: | chromosome 17 |
| Location: | 3550326 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g725450 | (1 of 1) K11789 - HIV-1 Vpr-binding protein (VPRBP, DCAF1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGTCACTGATGCAGCTGCTGCAGCCCAAG |
| Internal bar code: | TGGGGCACTTCGCCAGGGAGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 235 |
| LEAP-Seq percent confirming: | 93.75 |
| LEAP-Seq n confirming: | 105 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCAAGTGATGCCTACAAGC |
| Suggested primer 2: | AGACTCAACGAGCAAACCGT |